PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Leptadeniahastata (Pers.) Decne (Asclepiadaceae) is widely African plant species used in traditional medicine as an anti-venom, antihypertensive, anti-diabetic, analgesic and also used for catarrh and skin disease. The powdered leaves of L. hastatawas extracted with methanol using maceration method and the resulting crude methanol extract (ME) was suspended in water and fractionated into hexane (HF), chloroform (CF), ethylacetate (EF) and n-Butanol (BF). Preliminary phytochemical screening of ME revealed the presence of carbohydrates, alkaloids, flavonoids, anthraquinones, saponins, tannins, glycosides, steroids and triterpenes while (HF) revealed the presence of steroids and triterpenes nucleus. HF was step-wisely eluted in a silica gel packed column to afford ten fractions H1-H10 and fraction H4 was selected for further purification using Preparative Thin Layer Chromatography, with the solvent system of hexane: ethyl acetate (8.5 : 2.5). This resulted in the isolation of a whitish crystalline compound M1 (βSitosterol). The structure of the compound was elucidated using chemical test and spectroscopic techniques (IR, 1D & 2D-NMR) and by comparison with reference spectral data. The LD50 of the methanol extract (ME) and the LD99 of the Echisocellatus venom were estimated to be greater than 5000 mg/kg and 2.2 mg/kg respectively. Anti-venom studies suggest that ME possess significant anti-venom activity against Echisocellatusvenom both In vitro and In vivo. Analgesic effect was evaluated using acetic acid induced writhing in mice. The results showed that ME (150 mg/kg) decreased writhing response with 84.6 % inhibition. However, the extract when compared to control showed statistically significant difference at (p˂0.05)
Related Project Materials
ETHNOBOTANICAL SURVEY OF PLANTS USED FOR THE TREATMENT OF TYPHOID FEVER IN NIGERIA, AND EVALUATION OF ANTI-TYPHOID ACTIVITY OF TERMINALIA AVICENNOIDES GUILL & PERR. (COMBRETACEAE)
ABSTRACT
An ethnobotanical survey was conducted from December 2011 to April 2012 in Minna, Niger State, Nigeria. The survey was aimed at...
AN ASSESSMENT OF STAFF AND STUDENTS’ UTILIZATION OF ROUTINE MEDICAL CHECKUP IN AHMADU BELLO UNIVERSITY ZARIA, MEDICAL CENTRE, KADUNA STATE, NIGERIA
ABSTRACT
The study was the Assessment of Staff and Student’s Utilization of Routine Medical Checkup in Ahmadu Bello University Zari...
PROBLEM AND PROSPETS OF SMALL SCALE FISH FARMERS IN EDO STATE: A CASE STUDY OF OVIA NORTH EAST LOCAL GOVERNEMNT AREA OF EDO STATE
INTRODUCTION
The story of aquaculture in Nigeria is essentially the story of catfish culture and the hope of fish supply...
ANALYSIS OF THE IMPACT OF COURSE OF STUDY ON STUDENTS ATTITUDES TOWARDS ENTREPRENEURSHIP DEVELOPMENT IN NIGERIA (A STUDY OF HND II STUDENTS OF KADUNA POLYTECHNIC, KADUNA)
ABSTRACT
The study is an empirical work that attempted to investigate the backgrou...
THE ROLE OF INSURANCE COMPANY IN MANAGEMENT OF RISK IN MEDIUM SCALE ORGANIZATION (A Study of Usmer Water Ventures, Uyo)
ABSTRACT
The main purpose and focus of this study was to investigate and bring to limelight the roles of insurance c...
IMPACT OF CHILD ABUSE ON THE PARENTS AND THE PUBLIC
Background of child Study
Child abuse constitutes one of the most terrible crises of childhood. The occ...
ASSESSMENT OF TEACHERS’ AND STUDENTS’ ATTITUDES ON UTILIZATION AND MAINTAINANCE OF SCHOOOL FACILITIES IN SECONDARY SCHOOLS IN NIGERIA
Abstract
The study is entitled „Assessment of Teachers‟ and Students‟ Attitudes on Utilization and Maintenance of School Facilities...
THE ROLE OF VOCATIONAL TRAINING IN SUPPORTING THE ELECTRIC VEHICLE INDUSTRY
ABSTRACT: The role of vocational training in supporting the electric vehicle industry is crucial for developing the necessary workforce to adv...
THE INFLUENCE OF OMO DETERGENT TELEVISION COMMERCIALS ON CONSUMER’S PATRONAGE OF THE PRODUCT (A CASE STUDY OF WARRI METROPOLIS)
Abstract
The study investigated the influence of Omo Detergent Television Commercials On Consumer&rsquo...
ADDRESSING INDUSTRY DEMAND FORECASTING IN VOCATIONAL CURRICULUM PLANNING
Abstract: Addressing industry demand forecasting in vocational curriculum planning is criti...
